Detection of Unculturable Fungi in Soil Regions of Aziz Awa in Sulaimani province

Unculturable and non-sporulting fungi represent a great challenge instudying biotrophic, endotrophic mycorrhizal fungal groups. In thisstudy collection of soil sample from region Aziz Awa in sulaimaniprovince using culture-dependent culture in-dependenttechniques for cultivation unculturable the purpose ofexpanding studies on biodiversity soil. Sabouraud DextroseAgar with supplement as pyridoxine by replicating master plate fromhigher dilution three tinny colonies (less than 1mm diameter)had grown medium after incubation 5-7 weeks at 280C but had notshown growth when replicated traditional mycological media (PDA,Czapack Dox Agar Dextrose Agar) were selected,purified, studied culturally microscopically identified bymolecular methods. Four specific primer sets (NSIF/ITS4r, NSIF/ LRIF,EF4F/ITS4r EF60F/ITS4r) used to amplify partial sequences offungal r RNA gene included ITS sequences. The ofthree clones aligned through BLASTN phylogenetic analysis inNCBI available GenBank database revealed higher scoresand identities Fusarium sp, Penicillium sp Uncultured soilfungus clone. These results led us consider viable butnon culturable (VBNC) like common phenomenon somebacterial species.